Genomic and physiological features of slow-growing chloroflexi
Abstract/Contents
- Abstract
- Fast growing organisms adapted to high substrate concentrations have almost exclusively been the focus of microbiology research and led us to discover what we know about microbial metabolism. However, growth in Nature is usually not as fast and substrate conditions not as ideal. This work is an attempt to study how microorganisms adapt to slow growth and starvation conditions. Dehalococcoides mccartyi, a member of the Chloroflexi phylum and obligate strictly anaerobic organohalide respirer, was grown in chemostats adjusting doubling times between 5 and 50 days. The influence of growth rate on the microorganism's cell cycle was investigated using flow cytometry in combination with mathematical modeling showing that all phases of the cell cycle increased quasi-proportionally with doubling time. D. mccartyi chemostat cultures were also subjected to three months of substrate starvation and their protein and ribosome abundance was monitored during the onset of starvation revealing a 66% decrease in ribosomal content after 1.5 month of starvation but a remarkable stability of the overall proteome composition. Finally, the Chloroflexi phylum, to which D. mccartyi belongs, is a highly diverse phylum with few isolated representatives and which is enriched in the marine subseafloor. Metagenomic reconstruction of the genomes of 31 marine subsurface Chloroflexi revealed the quasi-ubiquitous presence of a functional catabolic Wood-Ljungdahl pathway and oxidation pathways for H2 and diverse organic compounds, making a strong case for a heterotrophic acetogenic lifestyle in these organisms
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Fincker, Maeva |
|---|---|
| Degree supervisor | Spormann, Alfred M |
| Thesis advisor | Spormann, Alfred M |
| Thesis advisor | Criddle, Craig |
| Thesis advisor | Dekas, Anne |
| Degree committee member | Criddle, Craig |
| Degree committee member | Dekas, Anne |
| Associated with | Stanford University, Civil & Environmental Engineering Department. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Maeva Fincker |
|---|---|
| Note | Submitted to the Civil & Environmental Engineering Department |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Maeva Fincker
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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