Defining trafficking programs of human plasmablasts responding to tissue-specific immune challenge
Abstract/Contents
- Abstract
- Antibody secreting cells are generated in regional lymphoid tissues and traffic as plasmablasts (PB) via lymph and blood to target sites for local immunity. The PB, a transiently activated population of B cells, are present in low numbers in the peripheral blood of healthy individuals. After acute infection or in setting of active immune responses, however, the frequency of blood PB increases dramatically, peaking 7 to 9 days after immunization or the onset of symptom. PB localized to target tissues undergo further maturation into antibody-secreting plasma cells. For this tissue-specific migration, the homing signature is imprinted on activated lymphocytes during their development by local environmental signals, but the trafficking mechanisms of PB remain poorly characterized. Here, we used multi-parameter flow cytometry to define trafficking programs (TPs, combinations of adhesion molecules and chemoattractant receptors) of PB, and their imprinting in patients in response to localized infection or immune insults. Using single cell-based analysis, we show that TPs enriched after infection or autoimmune inflammation of mucosae correlate with sites of immune response or symptoms, with different TPs imprinted during small intestinal, colon, throat and upper respiratory immune challenge. PB induced after intramuscular or intradermal influenza vaccination, including flu-specific antibody secreting cells, display TPs characterized by the lack of mucosal homing receptors. PB isolated from healthy donors display diverse mucosa-associated TPs, consistent with homeostatic immune activity. Identification of TP signatures of PB may facilitate non-invasive monitoring of organ-specific immune responses.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Seong, Yekyung |
|---|---|
| Associated with | Stanford University, Program in Immunology. |
| Primary advisor | Butcher, Eugene |
| Thesis advisor | Butcher, Eugene |
| Thesis advisor | Habtezion, Aida |
| Thesis advisor | Robinson, William (William Hewitt) |
| Thesis advisor | Wu, Joy |
| Advisor | Habtezion, Aida |
| Advisor | Robinson, William (William Hewitt) |
| Advisor | Wu, Joy |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Yekyung Seong. |
|---|---|
| Note | Submitted to the Program in Immunology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Yekyung Seong
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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