Optogenetic control of neurons beyond the brain
Abstract/Contents
- Abstract
- Optogenetic tools have immense value in targeting genetically specified excitable cells with high temporal precision throughout the body, and have been used extensively to investigate circuits of the brain. Optogenetics has great potential to analyze the neural circuitry of the peripheral nervous system (PNS) and spinal cord in freely moving animals, but has been limited by the technical difficulties of delivering opsins and light to these heterogeneous systems. In this thesis, I present novel optogenetic tools for use throughout the nervous system in freely moving animals. Included are viral strategies to target opsins to cutaneous C-fiber nociceptors and peripheral motor neurons as well as methods to deliver light via optical fiber to peripheral nerves, and wirelessly to cutaneous nerve terminals, spinal cord, and motor cortex of the brain. Using these optogenetic tools, we investigated pain and motor control in awake and freely moving mice and rats and provided proof-of-concept for potential therapies for neuropathic pain and paralysis. This technology opens the door for therapeutic development and more advanced investigations of the nervous system.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Montgomery, Kate L |
|---|---|
| Associated with | Stanford University, Department of Bioengineering. |
| Primary advisor | Delp, Scott |
| Thesis advisor | Delp, Scott |
| Thesis advisor | Deisseroth, Karl |
| Thesis advisor | Poon, Ada Shuk Yan |
| Advisor | Deisseroth, Karl |
| Advisor | Poon, Ada Shuk Yan |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Kate L. Montgomery. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Kathryn Lyle Montgomery
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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