A master puppeteer : machinery and motives for toxoplasma's manipulation of host cells
Abstract/Contents
- Abstract
- Toxoplasma gondii is an obligate, intracellular parasite capable of causing severe infections of warm-blooded animals. Infection of a cell produces a unique niche for the parasite named the parasitophorous vacuole (PV) initially composed of host plasma membrane invaginated during invasion. The PV and its membrane (PVM) are subsequently decorated with a variety of parasite proteins allowing the parasite to optimally grow in addition to manipulate host processes. Studies have identified parasite effectors involved in these host manipulations at the PVM and in the host cell, but the filed still does not know the complete list of Toxoplasma effectors in the host cell, how the translocation machinery for these effectors function, or have a complete idea of host- parasite interactions at the PVM. To this end, the work presented in this dissertation addresses three important topics. First, the study presented in Chapter 1 follows up on a candidate protein originating in the rhoptries that was identified as being involved in regulating host c-Myc induction. From the same study that identified the dense granule protein MYR1, we dissect the role this rhoptry protein has in c-Myc induction and effector translocation across the PVM. Second, the cargo being translocated across the PVM and into the host cell has mostly been identified by targeted approaches. In Chapter 3, we approach this gap by using an unbiased proteomic method to generate a list of candidate proteins that may be translocated out of the PV and into the host cell. Lastly in Chapter 4, our lab previously generated a dataset of enriched host proteins at the PVM- host interface with MOSPD2 being a top hit. In this study we investigate the dynamics by which MOSPD2 associates with the PVM and the biological significance of this association. Collectively, these studies help us better understand the molecular machinery at the PVM and in the host cell that allow Toxoplasma to interact with its host cell.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Ferrel, Abel |
|---|---|
| Degree supervisor | Boothroyd, John C |
| Thesis advisor | Boothroyd, John C |
| Thesis advisor | Kim, Peter, 1958- |
| Thesis advisor | Monack, Denise M |
| Thesis advisor | Yeh, Ellen |
| Degree committee member | Kim, Peter, 1958- |
| Degree committee member | Monack, Denise M |
| Degree committee member | Yeh, Ellen |
| Associated with | Stanford University, Department of Microbiology and Immunology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Abel Ferrel. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/gf930gx6150 |
Access conditions
- Copyright
- © 2022 by Abel Ferrel
- License
- This work is licensed under a Creative Commons Attribution Non Commercial Share Alike 3.0 Unported license (CC BY-NC-SA).
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