Intracellular recording of single cell action potentials by nanoelectrodes
- Many biological cells use electricity to control their physical and chemical functions. Some cells do so by generating action potentials, which are rapid rises and falls in the electric potential difference across their cell membrane. In particular, muscle cells, neurons, and endocrine cells use action potentials to initiate mechanical contraction, perform signal processing, and trigger chemical release, respectively. Action potentials are generated by the rapid opening and closing of ion channels on the cell membrane. Malfunctions of these ion channels are the cause of many diseases and toxicity in humans. Therefore, understanding the electrophysiology and pathology of these cells requires accurate recording of their action potentials. In this thesis I will present our results in developing nanoelectrodes for accurate, minimally invasive, and high-throughput intracellular recording of single cell action potentials. Using mammalian cardiac cells as the target system, we first demonstrate intracellular recording by platinum nanopillar electroporation. We discover that the tight cell membrane-electrode interface allows recording with high signal-to-noise ratio. I will then move on to the development of a new nanoelectrode topology, namely iridium oxide nanotubes, which give more than an order of magnitude increase in recording duration. At the same time we also verify that our nanoelectrode recording is accurate compared to the patch clamp recording standard. Finally, I will present application of our nanoelectrodes to record human stem cell derived cardiomyotyes for drug screening assays and disease monitoring.
|Type of resource
|electronic; electronic resource; remote
|1 online resource.
|Lin, Ziliang Carter
|Stanford University, Department of Applied Physics.
|Cui, Yi, 1976-
|Cui, Yi, 1976-
|Statement of responsibility
|Ziliang Carter Lin.
|Submitted to the Department of Applied Physics.
|Thesis (Ph.D.)--Stanford University, 2014.
- © 2014 by Ziliang Lin
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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