Characterization of arterial-venous cell fate plasticity during coronary vessel development
Abstract/Contents
- Abstract
- Understanding arterial-venous fate plasticity is important for innovating and improving vascular regenerative therapies. During coronary vessel development in the mouse, coronary arteries originate from a venous vessel. In this study, single-cell RNA-seq was used to investigate the characteristics of the arterial-venous fate switch during coronary vessel development. Results showed that instead of rapidly losing all arterial-venous identity as soon as they exit the vein, nascent coronary cells coexisted in a continuum of gradual venous fate loss and arterial fate gain. A rare subpopulation of surprisingly mature pre-artery cells within this continuum crossed a transcriptional threshold to resemble mature arterial cells. These pre-artery cells were specified at an unexpectedly early developmental stage, and they were necessary for coronary arteriogenesis. Modulations of the cell cycle was found to be an important regulator of arterial-venous plasticity: activation of the cell cycle correlated with the loss of venous identity, while inhibition of cell cycle was necessary for arterial redifferentiation. The transcription factor COUP-TF2 inhibited the development of the pre-artery cells by activating the cell cycle.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Su, Tianying |
|---|---|
| Degree supervisor | Red-Horse, Kristy |
| Thesis advisor | Red-Horse, Kristy |
| Thesis advisor | Krasnow, Mark, 1956- |
| Thesis advisor | Simon, Michael, (Biology professor) |
| Degree committee member | Krasnow, Mark, 1956- |
| Degree committee member | Simon, Michael, (Biology professor) |
| Associated with | Stanford University, Department of Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Tianying Su. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Tianying Su
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