Mechanical regulation of E-cadherin adhesion and signaling in epithelia
Abstract/Contents
- Abstract
- Mechanical force has been shown to regulate the shape and size of tissues in multicellular organisms. Cells within the tissue can respond to mechanical stimuli and elicit a downstream signaling response called mechanotransduction. One of the many protein complexes that sense and respond to mechanical force in epithelial cells are E-cadherin cell-cell junctions. E-cadherin links neighboring cells, and through its interaction with catenin proteins, is a signaling hub for many pathways involved in cell proliferation, differentiation, and migration, and tissue morphogenesis. The focus of my thesis was to study how E-cadherin responds to changes in mechanical force and the role of tension in regulating downstream cellular processes. We showed that changes in mechanical force is balanced between both cell-cell junctions and cell-extracellular matrix (ECM) junctions in cell pairs. We also developed a cell stretching system as a technology for studying the role of mechanical force in epithelial tissue biology. We observed that mechanical stretch can regulate cell cycle progression through the phosphorylation and nuclear localization of β-catenin. Also uniaxial mechanical stretch can orient the mitotic spindle in the direction of stretch through the mechanosensitive E-cadherin/LGN complex. Altogether, my thesis provides more understanding of how cell-cell junctions respond to mechanical force and the role of mechanical force in E-cadherin-regulated cell cycle and cell division.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Hart, Kevin |
|---|---|
| Degree supervisor | Nelson, W. J. (W. James) |
| Thesis advisor | Nelson, W. J. (W. James) |
| Thesis advisor | Feldman, Jessica L |
| Thesis advisor | O'Brien, Lucy |
| Thesis advisor | Weis, William I |
| Degree committee member | Feldman, Jessica L |
| Degree committee member | O'Brien, Lucy |
| Degree committee member | Weis, William I |
| Associated with | Stanford University, Department of Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Kevin Hart. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Kevin Charles Hart
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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