Bioelectronic interfaces for molecular quantification
Abstract/Contents
- Abstract
- The rapid advances of Moore's law have empowered electronics to solve many of modern society's problems, with healthcare emerging as a promising new application for highly miniaturized instrumentation. More specifically, biomolecular analysis techniques used in clinical settings often rely on expensive benchtop instruments and skilled technicians to carry out diagnostic assays, limiting access to biomolecular data. Indeed, it would be preferable to develop molecular diagnostics that can be deployed in the field. Hoping to leverage the potential for integration offered by CMOS processing, there has been much interest in developing biosensors that can transduce molecular concentrations in electrical signals. However, outside of the continuous glucose monitor and a few others, developing these technologies for a broad spectrum of molecular targets has proven challenging. In this dissertation, I explore some candidate technologies for a broad bioelectronic molecular sensing platform. First, I discuss direct electrical detection of biomolecules and identify the fundamental sensitivity challenge they face, the electric double layer, as well as a few strategies to overcome it. I next describe the development of a custom molecular-electronic platform for field-effect biosensing and demonstrate some practical challenges facing this class of biosensors. After that, I apply some of lessons of direct electrical detection to a different, more robust biosensing technology: electrochemical aptamers. In the first project, I demonstrate how the sensitivity of an electrochemical aptamer can be enhanced by engineering the electric double layer. In the second project, I describe how further engineering of the double layer can be used to endow cross-reactive electrochemical aptamers with specificity. As a whole, these studies provide a new perspective on biomolecular- electronic transduction and make progress towards solving developing broadly useful bioelectronic molecular diagnostic platforms.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Kesler, Vladimir |
|---|---|
| Degree supervisor | Murmann, Boris |
| Thesis advisor | Murmann, Boris |
| Thesis advisor | Howe, Roger Thomas |
| Thesis advisor | Soh, H. Tom |
| Degree committee member | Howe, Roger Thomas |
| Degree committee member | Soh, H. Tom |
| Associated with | Stanford University, Department of Electrical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Vladimir Kesler. |
|---|---|
| Note | Submitted to the Department of Electrical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/fz837ms9068 |
Access conditions
- Copyright
- © 2022 by Vladimir Kesler
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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