Postsynaptic vesicular cycling and its role in local redistribution of surface receptors
Abstract/Contents
- Abstract
- The spatial distribution of membranes and receptors along neuronal dendrites, in both surface and internal compartments, is important for remodeling of dendritic morphology and receptive properties. Applying a hypertonic solution, a well-established method for evoking presynaptic vesicle release, to dendrites expressing pHluorin-tagged transferrin receptors, rapidly induces new dendritic protrusions enriched in those receptors. Kinetic studies reveal that the protrusion formation precedes the receptor enrichment, suggesting distinct steps in receptor redistribution. These protrusions likely form due to exocytosis, as their number is significantly reduced when SNARE-mediated fusion is blocked by tetanus toxin or botulinum A toxin. Receptor redistribution, on the other hand, likely occurs through both coupled endo- and exocytosis and surface diffusion, as receptor endocytosis and depletion are observed in the adjoining regions of the protrusions. Additionally, the frequency of induced protrusions depends on the degree of tonicity much like presynaptic release, although they form in non-synaptic regions and do not require glutamate or a rise in intracellular calcium. Moreover, neurons co-expressing tagged GluA1 and transferrin receptors show co-trafficking of both receptors into the protrusions induced by hypertonic solution. Taken together, these findings unveil a microdomain centered on dendritic protrusions, where synaptic resources such as key surface receptors, are drawn from nearby regions and gathered in those protrusions. Thus, this domain serves to enhance local response to signaling without altering global responsiveness.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Li, Li |
|---|---|
| Associated with | Stanford University, Neurosciences Program. |
| Primary advisor | Tsien, R. W. (Richard W.) |
| Thesis advisor | Tsien, R. W. (Richard W.) |
| Thesis advisor | Baccus, Stephen A |
| Thesis advisor | Malenka, Robert C |
| Thesis advisor | Shen, Kang, 1972- |
| Advisor | Baccus, Stephen A |
| Advisor | Malenka, Robert C |
| Advisor | Shen, Kang, 1972- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Li Li. |
|---|---|
| Note | Submitted to the Program in Neuroscience. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Li Li
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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