Immune development at single cell resolution
Abstract/Contents
- Abstract
- The immune system is highly complex and contains many different cell types that are able to recognize and fight invading pathogens. B cells and T cells are both part of the adaptive immune system and must undergo specialized training programs to specifically recognize non-self invaders. Because these immune cells must be able to recognize a wide variety of pathogens, these cells and the cells that train them are remarkably heterogeneous. During the past 10 years, single cell technologies have evolved at a rapid pace allowing for a deeper understanding of these immune cells at the single cell level. In this dissertation I explore three projects I pursued during my PhD, where I use a variety of single cell technologies to explore the immune education of both B and T cells. In chapter 2, I combine single cell RNA-sequencing with lineage tracing and ablation and recovery of cells to explore the lineage relationships between medullary thymic epithelial cells, the cells responsible for negative selection of self-reactive T cells. In chapter 3, I combine single cell RNA-sequencing, single cell ATAC-sequencing, and single cell antibody capture (CITE-sequencing) to explore the transcriptional and chromatin landscape of B cells during affinity maturation, the process during which B cells adapt to be highly specific to a single pathogen. In chapter 4, I use single cell RNA-sequencing to explore the heterogeneity of thymic tuft cells, a subpopulation of medullary thymic epithelial cells. Taken together, these chapters improve our understanding of events that occur during the education of B and T cells
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Wells, Kristen Lynn |
|---|---|
| Degree supervisor | Steinmetz, Lars |
| Thesis advisor | Steinmetz, Lars |
| Thesis advisor | Baker, Julie, (Professor of genetics) |
| Thesis advisor | Bassik, Michael |
| Thesis advisor | Greenleaf, William James |
| Degree committee member | Baker, Julie, (Professor of genetics) |
| Degree committee member | Bassik, Michael |
| Degree committee member | Greenleaf, William James |
| Associated with | Stanford University, Department of Genetics |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Kristen Wells |
|---|---|
| Note | Submitted to the Department of Genetics |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Kristen Lynn Wells
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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