Characterizing Toxin-Induced Neuronal Cell Death in Zebrafish
Abstract/Contents
- Abstract
- Zebrafish larvae are an ideal model organism for modeling spinal cord injury (SCI) and the subsequent biochemical response because of the unique ability to regenerate. Current methods to model injury suffer from a lack of cell specificity and reproducibility. By combining chemical and genetic tools, we can ablate cells in a spatiotemporal specific manner to model injury in the organism, as well as study regeneration. We developed a genetic method of neural ablation where SCI in zebra sh is initiated using encoded toxins, speci cally viral protein M2(H37A) and Nitroreductase (NTR), that are expressed within the CNS during development. Characterization of these toxins show that M2(H37A) kills more rapidly that NTR and leads to tissue loss throughout the CNS. Based on the speed and damaged caused by M2(H37A) we believe the viral toxin kills through necrosis, inducing apoptotic death in the surrounding cells due to activated Caspase-3 and TUNEL staining. A complete death and regeneration pro le following expression of M2(H37A) and NTR will develop a powerful model of SCI that can be applied widely. Transgenic zebra sh serve as a resource for studies investigating cell death and regeneration; and due to the severe and rapid ablation of M2(H37A) through a compound death mechanism, the toxic protein can be a tool of choice in injury modeling studies in a variety of model organisms.
Description
Type of resource | text |
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Date created | April 30, 2018 |
Creators/Contributors
Author | Piza, Patrick Anthony |
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Advisor | Mruk, Karen |
Advisor | Chen, James K. |
Subjects
Subject | Stanford University |
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Subject | Chemical Engineering |
Subject | ChEM-H |
Subject | Chemical & Systems Biology |
Subject | Developmental Biology |
Subject | Zebrafish |
Subject | Model Organism |
Subject | SCI |
Subject | CNS |
Subject | M2 |
Subject | NTR |
Subject | Apoptosis |
Subject | Necrosis |
Subject | In situ hybridization |
Subject | Honors Thesis |
Genre | Thesis |
Bibliographic information
Related Publication | Mruk, K. P., P.A.; Alnaquib; M.A.; Chen, J.K., Optogenetic ablation in zebrafish larvae: a new tool for CNS studies. In Progress 2018. |
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Location | https://purl.stanford.edu/fw599sf0356 |
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Preferred citation
- Preferred Citation
- Piza, Patrick. (2018). Characterizing Toxin-Induced Neuronal Cell Death in Zebrafish. Stanford Digital Repository. Available at: https://purl.stanford.edu/fw599sf0356
Collection
Undergraduate Honors Theses - Chemical Engineering
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- Contact
- ppiza@alumni.stanford.edu
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