Dynamics of translation termination and ribosome recycling
Abstract/Contents
- Abstract
- Translation is the final checkpoint of gene expression during which the genetic information in messenger RNA (mRNA) is converted into protein by the ribosome. One crucial piece of information encoded in the mRNA, the stop codon, instructs the ribosome to end protein translation once the full-length protein has been synthesized. The stop codon triggers arrival of release factors (RF) to promote release of the nascent protein from the ribosome (termination). Additional translation factors then bind to disassemble the ribosome off the mRNA (recycling). These protein factors facilitate the fast termination and recycling important for regular production of functional proteins and dysfunction of these stages of translation is linked to genetic diseases and cancer. Despite their importance, our current knowledge on the mechanism of even simple prokaryotic termination and recycling is incomplete. Here, I present my thesis work studying this mechanism in bacteria using single-molecule fluorescence. The simultaneous tracking of translation factor binding events and ribosome conformational changes during termination and recycling revealed the short-lived intermediates of termination and recycling that were previously elusive. Particularly, the restructuring of the ribosome that occurs during termination after class I RF catalyzes peptide release helped us understand how the ribosome is primed for the subsequent recycling of the complete translational machinery by three other factors (RRF, EF-G, IF3). These changes in the global conformation of the ribosome became the framework for the subsequent study of the mechanism of class II RF
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Prabhakar, Arjun |
|---|---|
| Degree supervisor | Puglisi, Joseph D |
| Thesis advisor | Puglisi, Joseph D |
| Thesis advisor | Bryant, Zev David |
| Thesis advisor | Sarnow, P. (Peter) |
| Degree committee member | Bryant, Zev David |
| Degree committee member | Sarnow, P. (Peter) |
| Associated with | Stanford University, Biophysics Program. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Arjun Prabhakar |
|---|---|
| Note | Submitted to the Biophysics Program |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Arjun Prabhakar
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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