Host-centric proteomics of stool : monitoring global host responses to the gut microbiota
Abstract/Contents
- Abstract
- The diverse community of microbes that inhabits the human bowel, known as the gut microbiota, is vitally important to human health. While the identity and abundance of the microbes has been widely studied, little is known about the ways in which they interact with their host. The breakdown of the host-microbiota symbiosis has been linked to numerous diseases, including inflammatory bowel diseases, autoimmune diseases and even autism. Host-expressed proteins are essential for maintaining this mutualistic relationship and serve as reporters on the status of host-microbiota interactions. Previously, a global means for non-invasively monitoring the expression of proteins by the host in the gastrointestinal tract has not been described. In this thesis, I elucidate the need for an unbiased method to monitor host responses to the microbiota and describe a mass spectrometry-based method we developed, called host-centric proteomics of stool, which can simultaneously measure hundreds of host secreted proteins. I then illustrate how this method can be used to study host protein secretions in various regions of the gastrointestinal tract and the host responses to antibiotic-associated pathogens.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Lichtman, Joshua Sturgess |
|---|---|
| Associated with | Stanford University, Department of Chemical and Systems Biology. |
| Primary advisor | Elias, Joshua |
| Thesis advisor | Elias, Joshua |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Relman, David A |
| Thesis advisor | Sonnenburg, Justin, 1973- |
| Thesis advisor | Wandless, Thomas |
| Thesis advisor | Wysocka, Joanna, Ph. D |
| Advisor | Khosla, Chaitan, 1964- |
| Advisor | Relman, David A |
| Advisor | Sonnenburg, Justin, 1973- |
| Advisor | Wandless, Thomas |
| Advisor | Wysocka, Joanna, Ph. D |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Joshua Sturgess Lichtman. |
|---|---|
| Note | Submitted to the Department of Chemical and Systems Biology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Joshua Sturgess Lichtman
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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