The nucleus serves as the pacemaker for the cell cycle
Abstract/Contents
- Abstract
Mitosis is a dramatic cellular process that affects all parts of the cell. In Xenopus embryos and extracts it is driven by the activation of a bistable trigger circuit, whose various components are localized in the nucleus, centrosome, and cytoplasm. In principle, whichever cellular location has the fastest intrinsic rhythm should act as a pacemaker for the process. Here 15we followed tubulin polymerization and depolymerization in Xenopuse gg extracts supplemented with demembranated sperm, and thereby identified locations where mitosis first occurred. We found that mitosis was commonly first initiated at sperm-derived nuclei and their accompanying centrosomes, and then spread outward in circular trigger waves.The cell cycle was~20% more rapid at the nucleus/centrosome-associated trigger wave sources than in the regions of the extract 20that appeared not to be entrained by trigger waves. Nuclei produced from phage DNA, which did not possess centrosomes, also acted as trigger wave sources, but purified centrosomes in the absence of nuclei did not. We conclude that the nucleus accelerates mitotic entry and propose that it acts as a pacemaker for cell cycle.
This data collection contains the raw data and code that was used to process the raw data that were used to derive the conclusion that was made above.
Description
Type of resource | software, multimedia |
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Date created | 2020 |
Creators/Contributors
Author | Afanzar, Oshri | |
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Principal investigator | Ferrell, James E |
Subjects
Subject | Department of Chemical and System Biology |
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Subject | Stanford Medicine |
Genre | Dataset |
Bibliographic information
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- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under an Open Data Commons Public Domain Dedication & License 1.0.
Collection
Stanford Research Data
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- afanzar@stanford.edu
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