Temporal profiling of Salmonella transcriptional dynamics during macrophage infection using a comprehensive reporter library
- The transcriptome of Salmonella enterica serovar Typhimurium (S. Tm) dynamically responds to the rapid environmental shifts intrinsic to S. Tm lifestyle, such as entry into the Salmonella-containing vacuole (SCV) within macrophages. Intracellular S. Tm must respond to the acidity of the SCV, accumulation of reactive oxygen/nitrogen species, and fluctuations in nutrient availability. Here, we construct a comprehensive library of GFP-reporter strains representing ~3,000 computationally identified S. Tm promoters to study the dynamics of transcriptional regulation. We quantified promoter activity during in vitro growth in defined and complex media and during intracellular infection of RAW 246.7 macrophages. Using bulk measurements and single-cell imaging, we uncover medium-specific transcriptional regulation and population-level heterogeneity in the activity of virulence-related promoters, and previously unidentified transcriptional activity of genes related to metabolism, metal homeostasis, and canonical pathogenicity islands during infection. Along with the library, these data sets provide a powerful resource for systems-level interrogation of Salmonella transcriptional dynamics.
|Type of resource
|July 21, 2023; September 13, 2023; September 13, 2023
|July 20, 2023
|Research team head
|Research team head
|Huang, Kerwyn C.
|transcriptional reporter fusion
|in vitro screening
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- This work is licensed under a Creative Commons Attribution 4.0 International license (CC BY).
- Preferred citation
- Nguyen, T., Diaz, O., Monack, D., and Huang, K. (2023). Temporal profiling of Salmonella transcriptional dynamics during macrophage infection using a comprehensive reporter library. Stanford Digital Repository. Available at https://purl.stanford.edu/fc012fq3845. https://doi.org/10.25740/fc012fq3845.
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