Microfluidics meet ionizing radiation : drop-based flow radiocytometry and liquid sheet jet-based for X-ray spectroscopy
Abstract/Contents
- Abstract
- This thesis is focused on the development of two microfluidic platforms for multiphase sample delivery in assays involving ionizing radiation. In the first part of the work, a water-in-oil droplet microfluidic platform was designed and built for single-cell radiopharmaceutical studies. Radiotracers are widely used to track molecular processes with high sensitivity and specificity. However, most radiotracer detection methods have spatial resolution inadequate for single-cell analysis and cannot probe cellular heterogeneity, one of the most important challenges in cancer therapeutics. Here we introduce a robust, high-throughput single-cell radiometry based on radiofluorogenesis and droplet optofluidics. As a proof-of-concept application, we quantified [18F]fluorodeoxyglucose radiotracer uptake in single human breast cancer cells to assess glucose metabolism at the single-cell level. The second multiphase sample delivery system is a microfluidic nozzle designed to create liquid jet in air for X-ray spectroscopy analysis. We created stable free-surface liquid sheet jets with a width and length on the order of millimeters and sheet thickness on the order of microns and even sub-micron. These large aspect ratios provide a large target for the X-ray beam while minimizing background signal associated with line-of-sight integration of the detection. We conducted an experimental and numerical study to understand how fluid properties, pressure, momentum fluxes, surface tension, and nozzle geometry all interact to determine flow structures. The work developed scaling theories that predict sheet jet thickness, width, and length strictly from nozzle geometry, flow rate, and thermophysical fluid parameters. Interestingly, given this scaling analysis, no computer-aided simulations are needed for accurate flow structure prediction. Together the device design and theories provide a clear path to the rapid and efficient design of liquid sheet jets.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Ha, Byunghang |
|---|---|
| Degree supervisor | Pratx, Guillem |
| Degree supervisor | Santiago, Juan G |
| Thesis advisor | Pratx, Guillem |
| Thesis advisor | Santiago, Juan G |
| Thesis advisor | Tang, Sindy (Sindy K.Y.) |
| Degree committee member | Tang, Sindy (Sindy K.Y.) |
| Associated with | Stanford University, Department of Mechanical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Byunghang Ha. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/dy744ys8758 |
Access conditions
- Copyright
- © 2021 by Byunghang Ha
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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