Techniques for a chip-in-cell wireless platform for single cell monitoring
Abstract/Contents
- Abstract
- The rapid growth and development of technology has significant implications for healthcare, personalized medicine, and our understanding of biology. In this work, we follow the design and implementation of multiple subsystems of a larger vision of a non- invasive, intracellular wireless platform. Our vision is to develop a chip in a cell (CHIC) platform. This work details the journey toward the first demonstration of wireless detection and communication of an electronic device inside a cell, the fabrication of a microfluidic device to enhance the throughput of the wireless system, and the work toward integrating the subsystems together into the CHIC platform. The intracellular wireless platform is a multidisciplinary concept and requires expertise and work in a variety of topics. Specifically, we first demonstrate that a 25 μm wireless radio frequency identification (RFID) device can be uptaken by a mouse melanoma cell and detected and identified externally while located intracellularly. The performance of the electronic communication system is quantified before and after immersion in a biological environment, and the results show distinct electrical responses for different RFID designs, allowing for classification of a RFID from looking at the electrical output. Second, we show a robust method to fabricate a polydimethyliloxane (PDMS) microfluidic device with critical channel features located near the periphery. The fabricated device has a window cutout, allowing close placement and integration of the channel with solid state circuits and devices. Third, we detail the work toward integrating the wireless subsystem and PDMS device to realize our non-invasive, intracellular wireless platform. We show preliminary results of the complete system and describe the design process so that future generations can further refine the system. This work precedes and facilitates the development of long term intracellular measurement systems for personalized medicine and furthering our understanding of intrinsic biological behaviors. It helps provide an advanced technique to better assess the long term evolution of cellular physiology as a result of drug and disease stimuli in a way that is not enabled by current solutions.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Yang, Mimi Xuan |
|---|---|
| Degree supervisor | Wong, Hon-Sum Philip, 1959- |
| Thesis advisor | Wong, Hon-Sum Philip, 1959- |
| Thesis advisor | Akin, Demir |
| Thesis advisor | Poon, Ada Shuk Yan |
| Degree committee member | Akin, Demir |
| Degree committee member | Poon, Ada Shuk Yan |
| Associated with | Stanford University, Department of Electrical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Mimi Xuan Yang. |
|---|---|
| Note | Submitted to the Department of Electrical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Mimi Xuan Yang
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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