Development and application of new optical methods for the study of molecular motor regulation for multimotor transport in live cells
Abstract/Contents
- Abstract
- Life is made possible by 1) making complex biological macromolecules which then have to 2) arrive at specific physical locations. This second transport step is critical and becomes increasingly difficult as cells become large as is the case with eukaryotic cells. The problem of transport is solved in nature by the development of active intracellular transport machinery. The base units of this transport are molecular motors which are subject to many layers of regulation to properly transport cargos to where they need to function in the cell. For long range transport the primary motors are dynein and kinesin, which can walk along microtubules for long distances. Both these motors have been studied in purified systems but our understanding of how they work in cells is still lacking. I present work developing and using new optical methods to study the dynamics of motor activity in live neurons. First, Nanoparticle assisted optical tethering of endosomes (NOTE) is developed to show that kinesin and dynein, when active, are mechanically similar in the context of axons. Second, multipolarization darkfield microscopy is developed to track the orientation of endosomes as they are transported. Combination of orientation and position tracking allows inferences into the dynamics of the motor team transporting the cargo.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Kaplan, Luke |
|---|---|
| Associated with | Stanford University, Department of Biophysics. |
| Primary advisor | Cui, Bianxiao |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Brünger, Axel T |
| Thesis advisor | Shen, Kang, 1972- |
| Thesis advisor | Theriot, Julie |
| Advisor | Brünger, Axel T |
| Advisor | Shen, Kang, 1972- |
| Advisor | Theriot, Julie |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Luke Kaplan. |
|---|---|
| Note | Submitted to the Department of Biophysics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Luke Kaplan
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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