Epigenetic regulation of olfactory receptor gene expression by the Drosophila Myb-MuvB/dREAM complex
Abstract/Contents
- Abstract
- In both mammals and insects an olfactory neuron will usually select a single olfactory receptor and repress remaining members of large receptor families. Here we show that a conserved multiprotein complex Myb-MuvB(MMB)/dREAM plays an important role in mediating neuron-specific expression of the CO2 receptor genes (Gr63a/Gr21a) in Drosophila. Activity of Myb in the complex is required for expression of Gr63a/Gr21a and it acts in opposition to the histone methyltransferase Su(var)3-9. Consistent with this, we observe repressive di-methylated H3K9 modifications at the receptor gene loci suggesting a mechanism for silencing receptor gene expression. Conversely, other complex members, Mip120 and E2F2, are required for repression of Gr63a in inappropriate neurons. Misexpression in mutants is accompanied by an increase in the H3K4me3 mark of active chromatin at the receptor gene locus. Nuclei of CO2 receptor-expressing neurons contain reduced levels of the repressive subunit Mip120 compared to surrounding neurons and increased levels of Myb, suggesting that activity of the complex can be regulated in a cell-specific manner. Our evidence suggests a model in which olfactory receptors are regulated epigenetically and the MMB/dREAM complex plays a critical role in specifying, maintaining and modulating the receptor-to-neuron map.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Sim, Choon Kiat |
|---|---|
| Associated with | Stanford University, Department of Genetics |
| Primary advisor | Lipsick, Joseph Steven, 1955- |
| Thesis advisor | Lipsick, Joseph Steven, 1955- |
| Thesis advisor | Fuller, Margaret |
| Thesis advisor | Luo, Liqun, 1966- |
| Thesis advisor | Sidow, Arend |
| Advisor | Fuller, Margaret |
| Advisor | Luo, Liqun, 1966- |
| Advisor | Sidow, Arend |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Choon Kiat Sim. |
|---|---|
| Note | Submitted to the Department of Genetics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Choon Kiat Sim
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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