Proximal proteomics identifies novel subcellular localization and protein-protein interactions in Raf1-dependent cancers and SARS-CoV-2 infection
Abstract/Contents
- Abstract
- Protein-protein interactions are essential to communication of information within cells. These interactions are altered in response to extracellular stimuli, intracellular differences in the presence of metabolites, and numerous other cell-state differences. Dysregulation of proteoforms and the complexes they form occurs in any disease state and restoring appropriate function to cells requires understanding how these signaling pathways and quaternary protein structures are disrupted. To understand the dependence of various tumors on the Raf-1 protein, proximity proteomics strategies were used. These experiments identified a novel mitochondrial localization of Raf-1 and an interaction with glutaminase. To better understand this localization and novel interaction, experiments that force Raf-1 localization to various compartments are used to decouple its canonical MAPK pathway capability and the novel glutaminase-altering function and further demonstrate its importance for cancer. Beyond tumorigenesis, protein-protein interactions are also critical for viral infection and pathogenesis. To understand these processes, BioID proteomics was performed on 17 SARS-CoV-2 proteins. This work identified several pathways by which SARS-CoV-2 suppressed cellular immunity, novel subcellular localization for some of the SARS-CoV-2 proteins, and points of difference between CoV-2 and other coronaviruses.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2024; ©2024 |
| Publication date | 2024; 2024 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Shanderson, Ronald Leslie |
|---|---|
| Degree supervisor | Khavari, Paul A |
| Thesis advisor | Khavari, Paul A |
| Thesis advisor | Ferrell, James Ellsworth |
| Thesis advisor | Jackson, Peter K. (Peter Kent) |
| Thesis advisor | Oro, Anthony, 1958- |
| Degree committee member | Ferrell, James Ellsworth |
| Degree committee member | Jackson, Peter K. (Peter Kent) |
| Degree committee member | Oro, Anthony, 1958- |
| Associated with | Stanford University, School of Medicine |
| Associated with | Stanford University, Program in Cancer Biology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Ronald Leslie Shanderson III. |
|---|---|
| Note | Submitted to the Program in Cancer Biology. |
| Thesis | Thesis Ph.D. Stanford University 2024. |
| Location | https://purl.stanford.edu/df644bg7233 |
Access conditions
- Copyright
- © 2024 by Ronald Leslie Shanderson
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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