Ribosomes : stability, instability, and cellular elasticity
Abstract/Contents
- Abstract
- The ribosome is usually defined by what it does, what it's made of, or what it looks like. This thesis explores these definitions with the intention of forming a unified appreciation for how the composition of the protein biosynthesis apparatus relates to what it does in a cell. Understanding the compositional nature of ribosomes first requires defining them, and I do this by discussing the stability of the ribosome particle and the instability of associated factors that control the translation mechanism. This framework is critical for appreciating the first major direction of my dissertation research that is described in the first three chapters of this thesis. In these sections, I will describe several approaches to dissect eukaryotic translation at the single-molecule level that established the groundwork of a system to study for many years to come. In recent years, the stability of the mature ribosomal particle has been questioned, raising the possibility that such irregularities might relate to what it does via a ribosome "code". As I hope to demonstrate in the second major direction of my dissertation, there is an enormous gap between the "what it's made of" and "what it does" perspectives on the ribosome. Recognizing the centrality of the ribosome for cellular protein homeostasis is crucial to this end and sometimes necessitates thinking off the ribosome. This thesis will conclude with a discussion of broad implications and future directions that new researchers in the field may pursue.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Johnson, Alexander Geoffrey |
|---|---|
| Degree supervisor | Puglisi, Joseph D |
| Thesis advisor | Puglisi, Joseph D |
| Thesis advisor | Brandman, Onn |
| Thesis advisor | Jarosz, Daniel |
| Thesis advisor | Sarnow, P. (Peter) |
| Thesis advisor | Wandless, Thomas |
| Degree committee member | Brandman, Onn |
| Degree committee member | Jarosz, Daniel |
| Degree committee member | Sarnow, P. (Peter) |
| Degree committee member | Wandless, Thomas |
| Associated with | Stanford University, Department of Chemical and Systems Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Alexander Geoffrey Johnson. |
|---|---|
| Note | Submitted to the Department of Chemical and Systems Biology. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Alexander Geoffrey Johnson
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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