Deconvoluting the immune repertoire
Abstract/Contents
- Abstract
- In this thesis, I will demonstrate how combined functional/genomic analysis of the immune repertoire provides a clearer picture of a patient's immune response. I will show tools that I have advanced and developed for characterizing the immune system: immune repertoire library construction, long read sequencing on the Illumina platform, high throughput functional assays with antibodies cloned from single cells, and a data processing framework for combining functional and repertoire data. I will show influenza specific lineage recall over subsequent years of vaccination with trivalent influenza vaccine and identify several broadly binding anti-influenza antibody lineages. I will show how this platform can be used to identify which antibody lineages comprise segments of the mixed signal from a serum binding assay. Furthermore I will present evidence of low seroprevalence of influenza subtypes with H2, H5, H7 and H9 HA and apply this platform to identify multiple candidate antibodies for treating seasonal and pandemic influenza. Lastly I will show progress on a method for rapid DNA fabrication that could be used to quickly produce antibody genes mined from the repertoire, but has broader potential application in the field of synthetic biology.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Emig, Christopher J |
|---|---|
| Associated with | Stanford University, Department of Bioengineering. |
| Primary advisor | Quake, Stephen Ronald |
| Thesis advisor | Quake, Stephen Ronald |
| Thesis advisor | Bryant, Zev David |
| Thesis advisor | Fordyce, Polly |
| Advisor | Bryant, Zev David |
| Advisor | Fordyce, Polly |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Christopher J. Emig. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Christopher Joseph Emig
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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