Cryogenic single-molecule active control microscopy with fluorescent proteins
Abstract/Contents
- Abstract
- Superresolution microscopy is a powerful optical technique for studying molecules and structures within biological cells at size scales below the optical diffraction limit of ~200-300 nm. Performing superresolution imaging on samples maintained at cryogenic temperatures provides several advantages: reduced photobleaching resulting in improved localization precisions, the use of plunge-freezing as a chemical-free fixation technique, and the potential of correlative imaging with cryogenic electron tomography (cryoET). However, single-molecule superresolution methods such as PALM and STORM are difficult to implement in frozen samples due to the limited availability of fluorophores with access to efficient active control mechanisms at low temperatures. The work presented in this dissertation establishes a workflow for cryogenic fluorescence imaging compatible with subsequent cryoET and details our search for control over the emissive states of two fluorescent proteins, PAmKate and mApple, under cryogenic conditions.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Sartor, Annina Manuela |
|---|---|
| Degree supervisor | Moerner, W. E. (William Esco), 1953- |
| Thesis advisor | Moerner, W. E. (William Esco), 1953- |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Fayer, Michael D |
| Degree committee member | Cui, Bianxiao |
| Degree committee member | Fayer, Michael D |
| Associated with | Stanford University, School of Humanities and Sciences |
| Associated with | Stanford University, Department of Chemistry |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Annina M. Sartor. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/db167fy9326 |
Access conditions
- Copyright
- © 2023 by Annina Manuela Sartor
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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