Epigenomic interrogation of development and regeneration
Abstract/Contents
- Abstract
- Mammalian development comprises the highly coordinated and robust processes of cellular proliferation, specification, and functionalization to form the diverse tissues of the adult body. A single cell, containing DNA from the mother and the father, divides and gives rise to increasingly specified stem and progenitor cells at controlled times during development. This process is driven by changes not to the DNA sequence itself, but to the activity of the DNA within each cell, and the genes that are turned on and off, through modifications to the epigenome. During adult regeneration these processes can be reversed and repeated to re-build tissue that has been lost due to aging or injury. Our understanding of these processes has accelerated over the last decade, as next-generation sequencing technologies have made it possible to measure the state of the epigenome and gene expression in diverse cell types and organisms. Despite the progress that has been made, there are still challenges to understanding the dynamics of the epigenome in developmental processes. These include both experimental challenges of making measurements in small numbers of homogeneous primary cells, and computational challenges including mapping the maternal and paternal genomes separately and mapping repetitive sequences in the genome. In this dissertation, we employ a technique for mapping regions of accessible DNA in the genome, the Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq), to discover novel mechanisms of developmental allelic gene regulation, gene regulation in regeneration, and evolution of gene regulatory programs.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Carter, Ava Clayton |
|---|---|
| Degree supervisor | Chang, Howard Y. (Howard Yuan-Hao), 1972- |
| Thesis advisor | Chang, Howard Y. (Howard Yuan-Hao), 1972- |
| Thesis advisor | Greenleaf, William James |
| Thesis advisor | Oro, Anthony, 1958- |
| Degree committee member | Greenleaf, William James |
| Degree committee member | Oro, Anthony, 1958- |
| Associated with | Stanford University, Department of Stem Cell Biology and Regenerative Medicine. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Ava Clayton Carter. |
|---|---|
| Note | Submitted to the Department of Stem Cell Biology and Regenerative Medicine. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Ava Clayton Carter
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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