Development of CRISPR-based mouse models for scalable and quantitative in vivo functional cancer genomics
Abstract/Contents
- Abstract
- Large-scale genomic analyses of human cancers have catalogued somatic point mutations thought to initiate tumor development and sustain cancer growth. However, determining the functional significance of specific alterations remains a major bottleneck in our understanding of the genetic determinants of cancer. A diverse set of approaches—from genetic screens in cancer cell lines to the generation of defined alterations within organoids, allografts, and xenografts—have been used to study the function of genetic drivers of cancer. This thesis describes advances in cancer modeling that have stemmed from the implementation of genome-editing and next-generation sequencing in autochthonous mouse models of human cancer. Specifically, this dissertation focuses on the development and applications of higher-throughput mouse models that enable precise and quantitative analysis of the effects of multiplexed and/or combinatorial alterations on tumor growth in vivo. I discuss the potential impacts of these models on functional cancer genomics and outline the ways in which these models will improve our understanding of cancer. Finally, I speculate on additional innovations that will further increase the scope, precision, and value of in vivo models of human cancer.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Winters, Ian Paul |
|---|---|
| Degree supervisor | Winslow, Monte |
| Thesis advisor | Winslow, Monte |
| Thesis advisor | Fire, Andrew Zachary |
| Thesis advisor | Lipsick, Joseph Steven, 1955- |
| Thesis advisor | Sage, Julien |
| Degree committee member | Fire, Andrew Zachary |
| Degree committee member | Lipsick, Joseph Steven, 1955- |
| Degree committee member | Sage, Julien |
| Associated with | Stanford University, Department of Genetics. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Ian Paul Winters. |
|---|---|
| Note | Submitted to the Department of Genetics. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Ian Paul Winters
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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