Antibody adsorption at the silicone oil-water interface : exploring the strengths and limitations of the quartz crystal microbalance technique
Abstract/Contents
- Abstract
- High-concentration antibody formulations for parenteral delivery are increasingly packaged and stored in pre-filled glass syringes due to the ease of dosage control and administration. A thin film of silicone oil provides a lubricious layer to prevent freezing of the syringe plunger. This layer is thought to cause several complications when in contact with the antibody solution, namely surface-induced aggregation of the antibody and silicone oil droplet formation, leading to visible particle formation, increased viscosity of the solution, and possible unintended immunogenic reactions once injected into the patient. In this work, we describe an experimental platform to study the fate of antibodies on a model hydrophobic surface. By combining acoustic (quartz crystal microbalance with dissipation) and optical (ellipsometry) surface-sensitive techniques, we can monitor not only the amount of protein adsorbing onto a surface, but also its state of solvation and mechanical coupling to the surface. By this approach, we can examine the orientation of adsorbed proteins and detect aggregation on the surface. This platform allows us to study the effects of formulation conditions and excipients on antibody-surface interactions, with the ultimate goal of minimizing aggregate formation.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Wiseman, Meredith Elsa |
|---|---|
| Associated with | Stanford University, Department of Chemical Engineering |
| Primary advisor | Frank, C. W |
| Thesis advisor | Frank, C. W |
| Thesis advisor | Dunn, Alexander Robert |
| Thesis advisor | Swartz, James, 1955- |
| Advisor | Dunn, Alexander Robert |
| Advisor | Swartz, James, 1955- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Meredith Elsa Wiseman. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Meredith Elsa Wiseman
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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