Single-Cell Profile of Human Corneolimbus Reveals Cellular Diversity and Novel Putative Stem Cell Markers
Abstract/Contents
- Abstract
- The cornea and limbus compose the regenerative portion human ocular surface, whose function is critical to maintain clear vision. We present one of the first transcriptomic profiles of the human corneolimbus using single-cell RNA-sequencing data. Our data shows significant cellular diversity, with multiple distinct cell clusters representing multiple cell types. Expression of established biomarkers for differentiation, including KRT12, were higher in central corneal cell types versus limbal epithelia, potentially representing more differentiated and senescent states. The limbal epithelial clusters expressed known progenitor biomarkers, such as KRT14, KRT15, and ITGB1, but also expressed newly identified cell growth, migration, differentiation, and proliferation pathways. We identified SLC6A6 and TMEM158 as novel markers for transit amplifying cells in maintaining the limbal stem cell niche. Furthermore, we identified C7, SELE, and TCIM as novel markers specific to limbal stem cells. For all tissues, we utilized CytoTRACE, a novel computational framework that predicts differentiation state from the number of expressed genes per cell, to identify, in an unbiased fashion, stages of progenitor cells. This transcriptomics resource provides the first functional human ocular surface single-cell profile, serving as a foundation for future stem-cell based vision loss therapies.
Description
| Type of resource | text |
|---|---|
| Date created | June 2021 |
| Date modified | December 5, 2022 |
| Publication date | May 6, 2022 |
Creators/Contributors
| Author | Subramaniam, Varun Ramanan |
|---|---|
| Degree granting institution | Stanford University, Department of Biology, 2021 |
| Thesis advisor | Wu, Albert Y. |
| Thesis advisor | Morrison, Ashby |
Subjects
| Subject | Biology |
|---|---|
| Subject | Single Cell Sequencing |
| Subject | RNA Sequencing |
| Subject | Cornea |
| Subject | Limbus |
| Subject | Stem Cell |
| Subject | Transcriptomics |
| Subject | Ocular Surface |
| Genre | Text |
| Genre | Thesis |
Bibliographic information
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- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred citation
- Subramaniam, Varun Ramanan and Wu, Albert Y. and Morrison, Ashby. (2021). Single-Cell Profile of Human Corneolimbus Reveals Cellular Diversity and Novel Putative Stem Cell Markers. Stanford Digital Repository. Available at: https://purl.stanford.edu/bw190bf2187
Collection
Undergraduate Theses, Department of Biology, 2020-2021
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- Contact
- varsubr@stanford.edu
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