Uncovering dynamic behaviors of the adult drosophila intestine using novel imaging techniques
Abstract/Contents
- Abstract
- A major goal of modern biology is to observe and dissect dynamic behaviors. However dynamic biological behaviors occur over a vast range of timescales. Short-term (second to hour) events at the molecular and cellular scale often compound to drive long-term (days to years) processes making it inherently difficult to understand the full range of behaviors with a single technique. In addition, many organs of interest are encased within an opaque outer layer, making it challenging to monitor these dynamic processes in vivo. In this dissertation, I present two works that further the goal of monitoring dynamic biological behaviors within the intestines of living adult organisms. First, I will share a novel, intravital imaging technique called Bellymount. Using this technique, we performed the first longitudinal tracking experiments in the adult Drosophila intestine. With Bellymount we tracked stem cell lineage development and microbiota colonization in the same individual over multiple days. Second, I use an existing short-term continuous imaging technique along with fixed tissue analysis to understand how cell cycle progression may regulate adult stem cell fate outcomes.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Koyama, Leslie Ann Jaramillo |
|---|---|
| Degree supervisor | O'Brien, Lucy Erin, 1970- |
| Thesis advisor | O'Brien, Lucy Erin, 1970- |
| Thesis advisor | Fuller, Margaret T, 1951- |
| Thesis advisor | Kingsley, David M. (David Mark) |
| Thesis advisor | Nusse, Roel, 1950- |
| Degree committee member | Fuller, Margaret T, 1951- |
| Degree committee member | Kingsley, David M. (David Mark) |
| Degree committee member | Nusse, Roel, 1950- |
| Associated with | Stanford University, Program in Developmental Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Leslie Ann Jaramillo Koyama. |
|---|---|
| Note | Submitted to the Program in Developmental Biology. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Leslie Ann Jaramillo Koyama
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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