Discovery and engineering of terpenoid biosynthesis in plants
Abstract/Contents
- Abstract
- Plant terpenoids are structurally complex products with important and diverse sets of bioactivities. To date, the biosynthetic route to many terpenoids remains unknown. Elucidation of a complete biosynthetic pathway to terpenoids precludes engineering efforts for production in a heterologous host or efforts for in situ production in a different plant. We address this challenge through rapid gene screening in N. benthamiana, a heterologous plant host. Here, we worked on pathways and biology for three important targets: (1) momilactones from rice, an allelopathic molecule exuded by rice roots that inhibits the growth of neighboring plants, (2) limonoids from citrus, molecules with diverse biological properties, and (3) the chemotherapeutic taxanes from pacific yew. In addition to identifying minimum set of genes for pathway reconstitution, we have engineered a plant heterologous host for increased yields. These efforts enabled isolation and purification at milligram quantities of hard-to-access pathway products and derivatives and perform biological assays and structural elucidation.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | De La Peña Munoz, Ricardo |
|---|---|
| Degree supervisor | Sattely, Elizabeth |
| Thesis advisor | Sattely, Elizabeth |
| Thesis advisor | Abu-Remaileh, Monther |
| Thesis advisor | Spormann, Alfred M |
| Degree committee member | Abu-Remaileh, Monther |
| Degree committee member | Spormann, Alfred M |
| Associated with | Stanford University, Department of Chemical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Ricardo De La Peña. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/bs936hy4767 |
Access conditions
- Copyright
- © 2021 by Ricardo De La Pena Munoz
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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