Identification and characterization of human neural stem and progenitor cells
Abstract/Contents
- Abstract
- The prenatal human brain undergoes rapid development during the second trimester from a pool of neural stem cells (NSCs) known as radial glia (RG), which give rise to the neurons, oligodendrocytes, and astrocytes of the mature brain. We describe a method for the prospective isolation of multiple neural stem and progenitor cell (NSPC) populations from the developing human brain using various cell--surface markers that constitute their immunophenotype. The distinct NSPC populations include ventricular and outer radial glia (vRG, oRG), cells of excitatory and inhibitory neuronal lineages, oligodendrocyte precursor cells (OPCs), and astrocyte lineage cells. The purity of isolated NSPCs was assessed by correlating the expressed transcriptomes of individual index--sorted cells with their immunophenotype. We then confirmed the functional purity of isolated NSPCs regarding their lineage output potential through various in vitro differentiation assays, and through in vivo transplantation into the lateral ventricles of neonatal immunodeficient mouse brains. Histological analysis using human specific antibodies showed robust site--specific migration and engraftment of human NSPC subsets, which, after differentiation in vivo, gave rise to all three mature neural lineages in adult murine brains 6 months post--engraftment.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | He, Joy Qiyue |
|---|---|
| Degree supervisor | Weissman, Irving L |
| Thesis advisor | Weissman, Irving L |
| Thesis advisor | Andreasson, Katrin |
| Thesis advisor | Palmer, Theo |
| Thesis advisor | Wyss-Coray, Anton |
| Thesis advisor | Zuchero, J |
| Degree committee member | Andreasson, Katrin |
| Degree committee member | Palmer, Theo |
| Degree committee member | Wyss-Coray, Anton |
| Degree committee member | Zuchero, J |
| Associated with | Stanford University, School of Medicine |
| Associated with | Stanford University, Program in Stem Cell Biology and Regenerative Medicine |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Joy Qiyue He. |
|---|---|
| Note | Submitted to the Program in Stem Cell Biology and Regenerative Medicine. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/bs648gt0886 |
Access conditions
- Copyright
- © 2023 by Joy Qiyue He
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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