Identification and characterization of human neural stem and progenitor cells

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The prenatal human brain undergoes rapid development during the second trimester from a pool of neural stem cells (NSCs) known as radial glia (RG), which give rise to the neurons, oligodendrocytes, and astrocytes of the mature brain. We describe a method for the prospective isolation of multiple neural stem and progenitor cell (NSPC) populations from the developing human brain using various cell--surface markers that constitute their immunophenotype. The distinct NSPC populations include ventricular and outer radial glia (vRG, oRG), cells of excitatory and inhibitory neuronal lineages, oligodendrocyte precursor cells (OPCs), and astrocyte lineage cells. The purity of isolated NSPCs was assessed by correlating the expressed transcriptomes of individual index--sorted cells with their immunophenotype. We then confirmed the functional purity of isolated NSPCs regarding their lineage output potential through various in vitro differentiation assays, and through in vivo transplantation into the lateral ventricles of neonatal immunodeficient mouse brains. Histological analysis using human specific antibodies showed robust site--specific migration and engraftment of human NSPC subsets, which, after differentiation in vivo, gave rise to all three mature neural lineages in adult murine brains 6 months post--engraftment.


Type of resource text
Form electronic resource; remote; computer; online resource
Extent 1 online resource.
Place California
Place [Stanford, California]
Publisher [Stanford University]
Copyright date 2023; ©2023
Publication date 2023; 2023
Issuance monographic
Language English


Author He, Joy Qiyue
Degree supervisor Weissman, Irving L
Thesis advisor Weissman, Irving L
Thesis advisor Andreasson, Katrin
Thesis advisor Palmer, Theo
Thesis advisor Wyss-Coray, Anton
Thesis advisor Zuchero, J
Degree committee member Andreasson, Katrin
Degree committee member Palmer, Theo
Degree committee member Wyss-Coray, Anton
Degree committee member Zuchero, J
Associated with Stanford University, School of Medicine
Associated with Stanford University, Program in Stem Cell Biology and Regenerative Medicine


Genre Theses
Genre Text

Bibliographic information

Statement of responsibility Joy Qiyue He.
Note Submitted to the Program in Stem Cell Biology and Regenerative Medicine.
Thesis Thesis Ph.D. Stanford University 2023.

Access conditions

© 2023 by Joy Qiyue He
This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).

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