Part I: development of oligodeoxyfluorosides printed on paper for sensing food spoilage in the vapor, phase II: development of oligodeoxyfluorosides as sensor array for water-soluble toxic anions and pesticides
Abstract/Contents
- Abstract
- This thesis consists of two parts. In Part 1, DNA-based oligodeoxyfluoroside (ODF) dyes were developed to sense volatiles as they arise from food spoilage. In doing so, a new platform for fluorescence sensing was tested by inkjet printing of varied dyes on cellulose paper. ODFs are a class of short DNA-like oligomers with fluorophores replacing DNA bases. Six tetrameric ODFs of distinct sequences were printed on cotton paper using a commercial inkjet printer; printing methods and additives were evaluated for optimal fluorescence and response. ODFs were exposed via headspace volatiles to a variety of foods as they spoiled over a period of days. Fluorescence intensity and/or color changes during exposure to various foods were imaged under an epifluorescence microscope and the signals were quantified using digital values from red (R), blue (B), green (G), and luma (L) channels, creating "fingerprints" from the six sensor set and multiple color channels for each sensor. Using the digital data, some general trends were observed in food spoilage detection, such as different fluorescence changes according to food type, importance of spoiling conditions in fluorescence response due to influence from microbial activities, and the use of patterned response in differentiating spoiling foods and also distinguishing processes of spoilage in time course experiments. In Part 2, a new library of ODFs was synthesized for detection and differentiation of a set of target anions and pesticides in water. Detecting and quantifying anions or pesticides in water samples typically require complex instrumentation, adding cost and delaying analysis. These challenges were addressed by development of an optical molecular method to detect and discriminate a broad range of anionic and pesticides contaminants using a library of 1296 tetrameric-length ODFs composed of metal ligand and fluorescence modulating monomers on beads. These oligomers on beads were incubated with YIII or ZnII ions to provide affinity and responsiveness to anions and pesticides. An eight-ODF sensor set was able to differentiate seventeen anions at 250 μM in water from their patterns of response. Similarly, a ten-ODF sensor set was able to differentiate fourteen pesticides at 100 μM in water. Differential sensing through patterns of response was studied and used to develop applications where unknown concentrations or identity of samples were correctly predicted using statistical methods.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Kwon, Hyukin |
|---|---|
| Associated with | Stanford University, Department of Chemistry. |
| Primary advisor | Kool, Eric T |
| Thesis advisor | Kool, Eric T |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Advisor | Cui, Bianxiao |
| Advisor | Khosla, Chaitan, 1964- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Hyukin Kwon. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Hyukin Kwon
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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