Determination of the S. Cerevisiae INO80 Chromatin-Remodeling Complex in Mitotic Stability Pathways
Abstract/Contents
- Abstract
- The INO80 chromatin-remodeling complex was originally characterized as a transcriptional regulator that dynamically restructures chromatin, the packaging material of DNA. Recently, however, the complex has been implicated in chromosome segregation, as yeast deficient in INO80 subunits exhibit abnormal DNA ploidy. Currently, the precise mechanism by which the INO80 complex regulates mitotic stability remains unclear. This study sought to elucidate mechanisms for the INO80 complex in chromosome segregation by utilizing an integrative approach of genetics, molecular biology, and cell microscopy in S. cerevisiae. The hypothesis of this research is that the INO80 complex affects chromosome segregation by influencing microtubule dynamics. Through the assessment of genetic interactions between INO80 subunits and genes with characterized roles in mitosis, we find that INO80 subunits function in pathways related to kinesins, kinesin-like proteins, kinetochore-related, and cortical/microtubule associated proteins. Biochemical assays reveal a physical association between the INO80 complex and alpha-tubulin during G2/M-phase of the cell cycle. Cell microscopy provides further support for a role of the INO80 complex with microtubules, as fluorescently-labeled INO80 subunits localize to nuclear and cytoplasmic microtubules. Moreover, deletion of INO80 subunits results in an increased frequency of cells with shorter or undetectable cytoplasmic microtubules and non-elongated spindles. Interestingly, these results implicate the INO80 complex in microtubule function, which is important for kinesin (plus-end directed) and dynein (minus-end directed) motor proteins to generate the appropriate push/pull forces to separate sister chromatids, orient the spindle, and pull the nucleus into the daughter bud. This represents a novel mechanism by which INO80 regulates the critical mitotic machinery needed for chromosome segregation and to ensure accurate propagation of genetic materials into daughter cells.
Description
| Type of resource | text |
|---|---|
| Date created | June 15, 2014 |
Creators/Contributors
| Author | Chang, Christine | |
|---|---|---|
| Primary advisor | Morrison, Ashby | |
| Advisor | Stearns, Tim | |
| Degree granting institution | Stanford University, Department of Biology, 2014 |
Subjects
| Subject | mitosis |
|---|---|
| Subject | chromosome segregation |
| Subject | chromatin remodelers |
| Subject | INO80 |
| Subject | ARP5 |
| Subject | IES6 |
| Subject | spindle assembly |
| Subject | microtubules |
| Subject | microtubule motor proteins |
| Subject | S. cerevisiae |
| Subject | Biology |
| Subject | Stanford |
| Genre | Thesis |
Bibliographic information
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- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
- Chang, Christine, Morrison, Ashby, Stearns, Tim. (2014). Determination of the S. Cerevisiae INO80 Chromatin-Remodeling Complex in Mitotic Stability Pathways. Stanford Digital Repository. Available at: http://purl.stanford.edu/qn025cs1715
Collection
Undergraduate Theses, Department of Biology, 2013-2014
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- Contact
- cchang22@stanford.edu
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