Ablation of Quiescent Neural Stem Cells for Evaluation of Subsequent Repopulation
Abstract/Contents
- Abstract
- Recent work has established disruption of neurogenesis as a key cause of cognitive decline after use of brain irradiation to treat primary and metastatic tumors in children. Yet though this is widely accepted, little is known about the possibilities of restoration of normal neural stem cell (NSC) function after such a treatment, either through stem cell transplant or by promoting endogenous recovery by enhancing trophic effects. It is yet to be discovered whether endogenous quiescent neural stem cells (qNSCs) that are present in the irradiated brain have the potential to repopulate an injured neurogenic niche or whether resident stem cells are themselves damaged and are unable to repopulate the niche. It is also possible that a niche occupied by defective stem cells may simply block undamaged cells from occupying the niche. In this case, it may be necessary to ablate cells to create space for engraftment. Whereas most prior research has focused on solely anti-mitotic methods that spare rarely dividing quiescent NSCs, this research analyzes effective ablation of neural stem cells, including quiescent NSCs, in the dentate gyrus of the subgranular zone through the use of diphtheria toxin receptor-mediated cell death. My hypothesis was that partial ablation would show that qNSCs could repopulate the affected niche over time. After ablation treatment, it was observed that the number of nestin+ cells in the dentate gyrus (DG) was drastically reduced, and remained that way following a two-month recovery period. Along with this lack of renewal was partial ablation of neurogenesis in the olfactory bulb, evaluated by quantification of IdU+/CldU+ cells two months after ablation treatment. The data shown here provide new insight into the response of the neurogenic niche and surviving cells to effective ablation of local NSCs. This will potentially have a great impact on further research to be done regarding recovery procedures for irradiation-treated children.
Description
| Type of resource | text |
|---|---|
| Date created | June 15, 2014 |
Creators/Contributors
| Author | Rodriguez, Dominic | |
|---|---|---|
| Degree granting institution | Stanford University, Department of Biology, 2014 | |
| Primary advisor | Palmer, Theo | |
| Advisor | Jones, Patricia |
Subjects
| Subject | biology |
|---|---|
| Subject | neurosurgery |
| Subject | stem cells |
| Subject | quiescent |
| Subject | ablation |
| Genre | Thesis |
Bibliographic information
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
- Rodriguez, D., Palmer, T., & Jones, P. (2014). Ablation of Quiescent Neural Stem Cells for Evaluation of Subsequent Repopulation. Stanford Digital Repository. Available at: http://purl.stanford.edu/dz807gb9398
Collection
Undergraduate Theses, Department of Biology, 2013-2014
View other items in this collection in SearchWorksContact information
- Contact
- dominicr@stanford.edu
Also listed in
Loading usage metrics...