Development and characterization of osteosarcoma patient-derived xenograph models and investigation into the pathogenesis of osteosarcoma metastasis - Koehne, Amanda Lynn; Sweet-Cordero, Eric; Winslow, Monte; Bhutani, Nidhi; Giaccia, Amato J; Stanford University, Cancer Biology Program ([Stanford, California] : [Stanford University], 2019)
Osteosarcoma is the most common bone cancer of adolescents with 400 cases diagnosed annually in the United States. Early metastatic spread to the lungs is a hallmark feature of the disease, and is detrimental to overall survival of the patient. We used an approach to study osteosarcoma metastasis that involved the acquisition of patient tumors from both the primary site and metastatic site for whole genome and RNA sequencing, PDX production, and cell line production. Phenotypic characterization of the PDX and cell lines in vivo revealed that many will metastasize spontaneously and in a tail vein injection model. The function of ENPP1, a gene that is highly expressed in OS was also evaluated. ENPP1 expression correlated with metastatic capability in numerous OS cell lines. Gain and loss of function studies firmly established ENPP1's role in OS growth and metastasis. Our data described a role of ENPP1 in OS metastasis for the first time.
Submitted to the Cancer Biology Program.
Thesis Ph.D. Stanford University 2019.
Rights: (c) Copyright 2019 by Amanda Lynn Koehne
Rights: This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Keywords: theses; text