Characterizing the interaction between cell identity and cell-cycle regulation in the stomatal lineage of Arabidopsis
Abstract/Contents
- Abstract
- Asymmetric cell division is an important process that can link stem cell development to the cell cycle. Arabidopsis stomatal guard cell development, a model for plant stem cell lineages, requires asymmetric divisions and has been well characterized in terms of the distinct developmental stages and the transcription factors involved with each transition. To date, the effects or linkages of these developmental transitions on the cell cycle have not been defined in this system. Further, the existence and effects on the cell cycle of the putative plant DREAM complex, whose homologues regulate the cell cycle in animals, has not yet been demonstrated. In this thesis, I implemented two different methods of visualizing cell cycle stages in Arabidopsis leaves: a method that monitors nucleotide incorporation during DNA synthesis (EdU staining) and the CDT1A reporter line that marks the S and G2 stages of the cell cycle for live cell visualization. The latter method was used to begin to characterize how cell cycle timing is affected by differentiation or by members of the DREAM complex. Here, I present evidence that the critical transcription factors for stomatal development appear beginning in G1 and persist, likely with activity, through G2, as well as evidence that the DREAM complex is active from G1 through G2. To facilitate quantitative measurements of cell behaviors, I developed tutorials for MorphoGraphX, a software program that can segment and measure cell size, shape, and growth over time and that will be essential for continued studies of cell cycle and fate.
Description
| Type of resource | text |
|---|---|
| Date created | June 2017 |
Creators/Contributors
| Author | Cho, Nathan | |
|---|---|---|
| Primary advisor | Bergmann, Dominique | |
| Advisor | Walbot, Virginia | |
| Degree granting institution | Stanford University, Department of Biology, 2017 |
Subjects
| Subject | Biology |
|---|---|
| Subject | Cell Biology |
| Subject | Molecular Biology |
| Subject | Developmental Biology |
| Subject | Genetics |
| Subject | Plant Biology |
| Genre | Thesis |
Bibliographic information
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
- Cho, Nathan, Bergmann, Dominique, Walbot, Virginia. (2017). Characterizing the interaction between cell identity and cell-cycle regulation in the stomatal lineage of Arabidopsis. Stanford Digital Repository. Available at: http://purl.stanford.edu/pv279sn8486
Collection
Undergraduate Theses, Department of Biology, 2016-2017
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- Contact
- ncho2017@stanford.edu
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