Dynamic decoding of RNA
Abstract/Contents
- Abstract
- Genetic information encoded in messenger RNA (mRNA) is translated by the ribosome, a molecular machine that maps RNA to protein sequences following the genetic code. Translation of mRNA is regulated to control location, abundance, sequence and structure of the synthesized protein within the cell. Such regulation often involves pausing translation at a specific location within the mRNA, which may result in the local redefinition of the genetic code and synthesis of different protein products. In this dissertation, we investigate distinct pausing events involving actively translating ribosomes observed using single-molecule fluorescence spectroscopy. We particularly focus on the translational pauses induced by the chemical modification of RNA, the formation of stable RNA secondary structure and the nascent protein sequences, and how these lead to local redefinition of the genetic code. Finally, we discuss extension of the current experimental platform to understand how RNA elements induce pausing of different molecular machineries such as human immunodeficiency virus (HIV) reverse transcriptase, serving distinct roles in regulating RNA-based information transfer processes within the cell.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Choi, Junhong | |
|---|---|---|
| Degree supervisor | Puglisi, Joseph D | |
| Degree supervisor | Quake, Stephen Ronald | |
| Thesis advisor | Puglisi, Joseph D | |
| Thesis advisor | Quake, Stephen Ronald | |
| Thesis advisor | Bryant, Zev David | |
| Degree committee member | Bryant, Zev David | |
| Associated with | Stanford University, Department of Applied Physics. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Junhong Choi. |
|---|---|
| Note | Submitted to the Department of Applied Physics. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Junhong Choi
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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