The MHC class II cofactor, HLA-DM, interacts with immunoglobulin in B cells
Abstract/Contents
- Abstract
- B cells internalize antigen bound to membrane immunoglobulin and load antigen-derived peptides onto MHC class II proteins. How these pathways intersect is not well understood. We found that HLA-DM, a catalyst for peptide loading onto MHC class II, regulates immunoglobulin levels in human B cells and co-precipitates with immunoglobulin. In intact cells, HLA-DM and immunoglobulin are found in close proximity, and these complexes are in post-Golgi compartments. In vitro, in the endosomal pH range, the luminal domain of soluble HLA-DM (sDM) directly and specifically interacts with the immunoglobulin Fab domain, and sDM increases the amount of antigen released from immune complexes. These observations support a model in which HLA-DM facilitates the hand-off of antigen from immunoglobulin to MHC class II.
Description
Type of resource | text |
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Form | electronic; electronic resource; remote |
Extent | 1 online resource. |
Publication date | 2013 |
Issuance | monographic |
Language | English |
Creators/Contributors
Associated with | Strohman, Michael John | |
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Associated with | Stanford University, Program in Immunology. | |
Primary advisor | Mellins, Elizabeth | |
Thesis advisor | Mellins, Elizabeth | |
Thesis advisor | Davis, Mark M | |
Thesis advisor | Jardetzky, Theodore | |
Thesis advisor | Jones, Patricia P | |
Thesis advisor | Miklos, David (David B.) | |
Advisor | Davis, Mark M | |
Advisor | Jardetzky, Theodore | |
Advisor | Jones, Patricia P | |
Advisor | Miklos, David (David B.) |
Subjects
Genre | Theses |
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Bibliographic information
Statement of responsibility | Michael John Strohman. |
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Note | Submitted to the Program in Immunology. |
Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Michael John Strohman
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