A genetic screen to identify novel regulators of adult stem cell maintenance
Abstract/Contents
- Abstract
- Adult stem cells are an important class of cells responsible for the maintenance and regeneration of the body’s many tissues. These cells are under heavy investigation for potential therapeutic roles. However, one of the rate limiting steps is an understanding of the exact mechanisms and genes that regulate this class of cells. To better understand the genetic program regulating adult stem cells, a forward genetic screen was utilized involving a two-stage screening process comprising a Flp/FRT primary screen and an EGUF/hid secondary screen . Out of 3,118 mutant males produced, 1,041 were recovered, of which 412 showed loss of germ line clones and one showed overproliferation of germline clones in the primary screen. Of the 412 germ cell loss mutations, 59 passed through a secondary screen for cell lethality (EGUF/hid). 6 mutant strains have been partially or completely mapped, uncovering germ cell loss mutations in DNA Replication-Related Element Factor (DREF), Apoptosis Inducing Factor (AIF), Guanylyl Cyclase 32E (Gyc32E) and two other loci, as well as a germ cell overproliferation mutation in Star. Further research on DREF has shown that the allele we uncovered genetically separates DREF’s role in cell division and DNA-replication from its role in adult stem cell maintenance. Furthermore, I have uncovered a novel antagonistic interaction between DREF and members of the NuRD complex that is essential for the regulation of germline stem cell maintenance. These results suggest that our understanding of the genetic program of adult stem cells can still be enriched by well-designed, classic genetic screens.
Description
| Type of resource | text |
|---|---|
| Date created | June 15, 2014 |
Creators/Contributors
| Author | Angulo, Benjamin | |
|---|---|---|
| Primary advisor | Fuller, Margaret | |
| Advisor | Nelson, W James | |
| Degree granting institution | Stanford University, Department of Biology, 2014 |
Subjects
| Subject | Adult Stem Cells |
|---|---|
| Subject | DREF |
| Subject | Biology |
| Subject | Genetics |
| Subject | Developmental Biology |
| Subject | Stem Cells |
| Subject | Stanford University |
| Subject | Genetic Screen |
| Genre | Thesis |
Bibliographic information
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- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
- Angulo B, Bolival B, Srinivasan S, Nelson WJ, and Fuller MT (2014). A genetic screen to identify novel regulators of adult stem cell maintenance. Stanford Digital Repository. Available at: http://purl.stanford.edu/td546gw8911
Collection
Undergraduate Theses, Department of Biology, 2013-2014
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- Contact
- bangulo@stanford.edu
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