Design, synthesis, and evaluation of molecular transporters for probe, drug, and siRNA delivery
Abstract/Contents
- Abstract
- My graduate studies have focused on the design, synthesis, and biological evaluation of novel probe and drug delivery technologies. This research has explored the development of new molecular transporter scaffolds with a focus on step economy and translational costs as well as evaluation of their uptake and delivery properties in cells and animals. Chapter 1 provides a historical context and overview of guanidinium-rich molecular transporter technology. Chapter 2 describes the development of a new family of guanidinium-rich oligocarbonate molecular transporter which are flexibly and efficiently assembled by a one-step oligomerization strategy. These novel oligocarbonate transporters were shown to exhibit excellent uptake properties both in cells and animal models. Chapter 3 is directed at the utility of an oligomerization approach to generate molecular transporters by the design, synthesis, and evaluation of new aphipathic co-oligomers for the delivery of siRNA, an oligonucleotide cargo of intense therapeutic interest. Amphipathic carbonate co-oligomers were prepared by an oligomerization strategy and demonstrated to effectively package, deliver, and release functional siRNA in cells. Chapter 4 describes the effects of a branched guanidinium array on the transport and delivery efficiency of releasable dendrimeric guanidinium-rich transporters. These transporters were synthesized and demonstrated to deliver and release a small molecule for turnover by its intracellular target enzyme by bioluminescence assays in cells and transgenic animal models. Chapter 5 describes the design, synthesis, and preliminary biological evaluation of lipidated molecular transporter derivatives of the immunosuppressant drug rapamycin for topical delivery.
Description
Type of resource | text |
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Form | electronic; electronic resource; remote |
Extent | 1 online resource. |
Publication date | 2011 |
Issuance | monographic |
Language | English |
Creators/Contributors
Associated with | Cooley, Christina Barnes | |
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Associated with | Stanford University, Department of Chemistry | |
Primary advisor | Wender, Paul A | |
Thesis advisor | Wender, Paul A | |
Thesis advisor | Du Bois, Justin | |
Thesis advisor | Kool, Eric T | |
Advisor | Du Bois, Justin | |
Advisor | Kool, Eric T |
Subjects
Genre | Theses |
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Bibliographic information
Statement of responsibility | Christina Barnes Cooley. |
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Note | Submitted to the Department of Chemistry. |
Thesis | Thesis (Ph.D.)--Stanford University, 2011. |
Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Christina Barnes Cooley
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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