Single-molecule and super-resolution in living cells
Abstract/Contents
- Abstract
- Since the first successful detection single molecules over two decades ago, single-molecule spectroscopy has developed into a burgeoning field with a wealth of experiments at room temperature and inside living cells. Probing asynchronous and heterogeneous populations in situ, one molecule at a time, is not only desirable, but critical for many biological questions. Further, super-resolution imaging based on sequential imaging of sparse subsets of single molecules, has seen explosive growth within the last five years. This dissertation describes both the application of live-cell single-molecule imaging as an answer to important biological questions, and development and validation of fluorescent probes for targeted super-resolution imaging.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2011 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Lee, Hsiao-lu | |
|---|---|---|
| Associated with | Stanford University, Department of Chemistry | |
| Primary advisor | Moerner, W. E. (William Esco), 1953- | |
| Thesis advisor | Moerner, W. E. (William Esco), 1953- | |
| Thesis advisor | Fayer, Michael D | |
| Thesis advisor | Pecora, Robert, 1938- | |
| Advisor | Fayer, Michael D | |
| Advisor | Pecora, Robert, 1938- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Hsiao-lu Lee. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Ph. D. Stanford University 2011 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Hsiao-lu Lee
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