Characterizing sensory cell regeneration by facultative Lgr5+ cells in Mus musculus utricle
Abstract/Contents
- Abstract
- Recruitment of endogenous progenitors is critical during tissue repair. In the mammalian cochlea, damaged hair cells are not regenerated. As a result, hearing loss is permanent and negatively impacts the quality of life for over 48 million Americans. Adjacent to the cochlea in the inner ear are the vestibular organs responsible for our balance functions. Of the vestibular organs, the utricle requires mechanosensory hair cells to detect linear acceleration. After damage, non-mammalian utricles regenerate hair cells mitotically and non-mitotically. By contrast, mammalian utricles exhibit limited non-mitotic regeneration, with the source of regenerated hair cells currently unknown. Here, we used Lgr5-EGFP reporter mice to show that hair cell damage in neonatal mouse utricles activates the Wnt target gene, Lgr5, in supporting cells, which normally do not express Lgr5. Lineage tracing and time-lapse microscopy in utricles from Lgr5-EGFP-CreERT2; Rosa26R-tdTomato mice reveal that Lgr5+ cells non-mitotically become hair cell-like cells in vitro, a process termed “direct transdifferentiation.” Thus, Lgr5 marks damage-activated HC progenitors and may help reveal factors necessary for mammalian hair cell regeneration.
Description
Type of resource | text |
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Date created | June 15, 2014 |
Creators/Contributors
Author | Pham, Xuan Phien Nicole | |
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Degree granting institution | Stanford University, Department of Biology, 2014 | |
Primary advisor | Cheng, Alan | |
Advisor | Fernald, Russell |
Subjects
Subject | Biology |
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Subject | Otolaryngology |
Subject | regeneration |
Subject | Wnt |
Genre | Thesis |
Bibliographic information
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
- Pham, Xuan Phien Nicole. (2014). Stanford Digital Repository. Available at: http://purl.stanford.edu/rm461gj1082.
Collection
Undergraduate Theses, Department of Biology, 2013-2014
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- Contact
- xpham@stanford.edu
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