Transcriptional feedback links lipid synthesis to synaptic vesicle pools in Drosophila photoreceptors
Abstract/Contents
- Abstract
- Neurons can maintain stable synaptic connections across adult life. However, the signals that regulate expression of synaptic proteins in the mature brain are incompletely understood. Here we describe a transcriptional feedback loop between the biosynthesis of specific phospholipids containing long chain polyunsaturated fatty acids (LC-PUFAs) and the synaptic vesicle pool in adult Drosophila photoreceptors. Mutations that disrupt biosynthesis of these phospholipids cause degeneration of the axon terminal and loss of synaptic vesicles. While degeneration of the axon terminal is dependent on neural activity, activation of Sterol Regulatory Element Binding Protein (SREBP) is both necessary and sufficient to cause synaptic vesicle loss. Our studies demonstrate that SREBP regulates synaptic vesicle levels by controlling tetraspanins, critical organizers of membranous organelles. SREBP is an evolutionarily conserved regulator of lipid biosynthesis in non-neuronal cells; these studies reveal a surprising role for this feedback loop in maintaining synaptic vesicle pools in the adult brain.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Tsai, Jessica Whei | |
|---|---|---|
| Associated with | Stanford University, Neurosciences Program. | |
| Primary advisor | Clandinin, Thomas R. (Thomas Robert), 1970- | |
| Thesis advisor | Clandinin, Thomas R. (Thomas Robert), 1970- | |
| Thesis advisor | Barres, Ben | |
| Thesis advisor | Reimer, Richard J | |
| Thesis advisor | Wang, Xinnan | |
| Advisor | Barres, Ben | |
| Advisor | Reimer, Richard J | |
| Advisor | Wang, Xinnan |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jessica Whei Tsai. |
|---|---|
| Note | Submitted to the Program in Neurosciences. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Jessica Whei Tsai
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