Exploration of the regulatory networks of hybrid two-component systems in the model gut symbiont Bacteroides thetaiotaomicron
Abstract/Contents
- Abstract
- This dissertation describes the regulatory capabilities of hybrid two-component systems in the model gut bacteria Bacteroides thetaiotaomicron. These unique proteins are important for carbohydrate sensing and consumption in the Bacteroides, but the full extent of their regulatory networks has not been previously explored. We created a chimeric hybrid two-component system that fused the well-characterized sensing domain of one, BT1754, to the uncharacterized regulatory domain of another, BT0366. We then used the BT1754 ligand, fructose, to functionally activate BT0366. Using genome-wide transcriptional profiling, we discovered that BT0366 both upregulates adjacent genes associated with the degradation of the plant polysaccharide arabinan, but also downregulates distal genes involved in mucin consumption. Using Chromatin Immunoprecipitation with high throughput sequencing, we then found that BT0366 binds directly to the genomic loci it helps regulate. Next, we developed tools for live-cell fluorescent microscopy of Bacteroides thetaiotaomicron in order to allow us to describe the physical positioning of hybrid two-component systems and their partnering molecules. This dissertation both describes regulatory activities of hybrid two-component systems in this common gut microbe and also provides tools for further study of subcellular localization in Bacteroides species.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2014 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Lynch, Jonathan Braswell | |
|---|---|---|
| Associated with | Stanford University, Department of Microbiology and Immunology. | |
| Primary advisor | Sonnenburg, Justin, 1973- | |
| Thesis advisor | Sonnenburg, Justin, 1973- | |
| Thesis advisor | Amieva, Manuel | |
| Thesis advisor | Huang, Kerwyn Casey, 1979- | |
| Thesis advisor | Theriot, Julie | |
| Advisor | Amieva, Manuel | |
| Advisor | Huang, Kerwyn Casey, 1979- | |
| Advisor | Theriot, Julie |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jonathan Braswell Lynch. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2014. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2014 by Jonathan Braswell Lynch
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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