Droplet microfluidics platform for quantifying pathogens with single-cell resolution
Abstract/Contents
- Abstract
- Droplet microfluidics, where micro-droplets are suspended in an immiscible fluid, has enabled a wide range of biochemical applications. The objective of my thesis dissertation is to apply droplet microfluidics to pathogen detection. My thesis covers two parts. The first part involves the suppression of the cross-contamination of droplet content by using nanoparticles as the droplet stabilizer. The second part involves quantifying bacteria using droplet-based microfluidics with single cell resolution. In the first part of my dissertation, I show the development of amphiphilic nanoparticles (NPs) as an emulsion stabilizer to replace surfactants in droplet microfluidics. Compared with state-of-the-art surfactants, amphiphilic NPs not only exhibit comparable droplet stability but also mitigate the cross-contamination of droplet contents. The impact of this research is further validated by the licensing and commercialization of this method by a microfluidics company. In the second part of my dissertation, I present the design and application of droplet-based microfluidic systems to quantitatively detect bacteria with single-cell resolution. Compared with state-of-art bacterial detection methods, our droplet-based microfluidic method offers multiple advantages including higher sensitivity, higher specificity, and lower limit of detection (LOD). Further development of our method can enable rapid low-cost bacterial detection in a wide range of applications to prevent and combat infectious diseases.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Lyu, Fengjiao | |
|---|---|---|
| Degree supervisor | Tang, Sindy (Sindy K.Y.) | |
| Thesis advisor | Tang, Sindy (Sindy K.Y.) | |
| Thesis advisor | Andrews, Jason | |
| Thesis advisor | Chaudhuri, Ovijit | |
| Degree committee member | Andrews, Jason | |
| Degree committee member | Chaudhuri, Ovijit | |
| Associated with | Stanford University, Department of Mechanical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Fengjiao Lyu. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Fengjiao Lyu
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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